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The method is applicable to the determination of total α-galactosides and the estimation of raffinose in beet molasses, in juices and syrups from beet processing and beet sugar.
The raffinose present is hydrolysed by an enzyme to D-galactose which is then oxidised with nicotinamide-adenine dinucleotide (NAD): the NAD being reduced to NADH. The quantity of NADH formed is measured spectrophotometrically.
The absolute difference for results obtained under repeatability conditions should not be greater than 3 g/100 g.
Please click here to open ICUMSA Method GS4/8/1/2/3-18 (2017).