Home Scientific Article The decomposition of raffinose by a-Galactosidase an enzymatic reaction applied in the factory-process in japanese beet sugar factories

The decomposition of raffinose by a-Galactosidase an enzymatic reaction applied in the factory-process in japanese beet sugar factories

Takeo Yamane;

Raffinose in the final molasses was decomposed in the process of the factory

by OHgalaetosidase into sucrose and galactose, and many favourable results were

effected thereby. The new process and the results obtained are outlined as follows :

1. a-galactosidase was manufactured by culturing a mold, Mortierella vinacea.

2. Diluted final molasses was decomposed by the enzyme-containiing-mycelium of the mold, more than 60 % of raffinose in the molasses was decomposed. The used mycelium was repeatedly put in operation after washing.

3. The sucrose in the raffinose-decomposed-molasses was recovered by Steffen process and the saccharate obtained was used for main liming of the diffusion juice.

4. The crystal form of the 1st and 3rd sugars was improved, because the bad-form due to the existence of accumulated raffinose in the massecuites was avoided by the new process.

5. The beet-slicing capacity of the factory was increased, because the solid recoveries of boiled massecuite were highly increased.

6. The white sugar yield1 of the factory was limproved, because the discarding of the final molasses was entirely unnecessary and the sucrose from the decomposed raffinose also contributed thereto.

This new process has been adopted in three factories in Japan.

 

Proceedings: CITS 1971
Page: 187–194

Language: ENG

pdf download: CITS1971-187-194.pdf